Bio-Gel HT Hydroxyapatite from Bio-Rad

Product Specs
ItemBio-Gel HT Hydroxyapatite
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1300151
ModeHydroxyapaptite
TypeResins
Brand/TrademarkLSG PFTL

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) CLIPB4 Is a Central Node in the Protease Network that Regulates Humoral Immunity in Anopheles gambiae MosquitoesJournal of Innate ImmunitySeptember 13, 2023Xiufeng Zhang, Shasha Zhang, Junyao Kuang, Kathleen A. Sellens, Bianca Morejon, Sally A. Saab, Miao Li, Eve C. Metto, Chunju An, Christopher T. Culbertson, et al.buffer A (10 mm KPi, pH 6.8, 500 mm NaCl, 0.5 mm reduced l-glutathione), and dialyzed against buffer A. Bio-Gel HT Hydroxyapatite column (Bio-Rad) was utilized to separate proteins via a linear gradient of 10–100 mm KPi in buffer

(2) CLIPB4 is a central node in the protease network that regulates humoral immunity in Anopheles gambiae mosquitoesbioRxivJuly 20, 2023Xiufeng Zhang, Shasha Zhang, Junyao Kuang, Kathleen A. Sellens, Bianca Morejon, Sally A. Saab, Eve M Metto, Eve C. Metto, Chunju Osta, Christopher T. Culbertson, et al.buffer A (10 mM KPi, pH 6.8, 500 mM NaCl, 0.5 mM reduced L-Glutathione), and dialyzed against buffer A. Bio-Gel HT Hydroxyapatite column (Bio-Rad) was utilized to separate proteins via a linear gradient of 10-100 mM KPi in buffer

(3) The J Domain of Sacsin Disrupts Intermediate Filament AssemblyInternational journal of molecular sciencesDecember 12, 2022Dabbaghizadeh A, Paré A, Cheng-Boivin Z, Dagher R, Minotti S, Dicaire MJ, Brais B, Young JC, Durham HD, Gentil BJ.filters and solubilized NFL was then purified from the supernatant by affinity chromatography using Bio-gel® HT hydroxyapatite (130-0150, Bio-Rad, Mississauga, ON, Canada) and following the protocol of Leung and Liem [35]. The

(4) Structure of a human replisome shows the organisation and interactions of a DNA replication machineThe EMBO JournalDecember 1, 2021Morgan L Jones, Yasemin Baris, Martin R G Taylor, Joseph T P Yeelesglycerol, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% NP‐40‐S) for 90 min. Protein was applied to a 2 ml Bio‐Gel HT hydroxyapatite column (Bio‐Rad), and the flow through was re‐applied once. The column was first washed with 6 ml 50

(5) Antiviral RISC mainly targets viral mRNA but not genomic RNA of tospovirusPLOS PathogensJuly 28, 2021Hao Hong, Chunli Wang, Ying Huang, Min Xu, Jiaoling Yan, Mingfeng Feng, Jia Li, Yajie Shi, Min Zhu, Danyu Shen, et al.twice at 14,000 ×g at 4°C. Supernatant (40 mL) was loaded onto a 25×3.6 cm2 column packed with 50 mL Bio-Gel HT Hydroxyapatite (Bio-Rad, Hercules, CA). Chromatography separations were performed on ÄKTA avant 25 (GE Healthcare).

(6) IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and ConformationalFrontiers in ImmunologyJune 15, 2021Mirela Curin, Huey-Jy Huang, Tetiana Garmatiuk, Sandra Gutfreund, Yvonne Resch-Marat, Kuan-Wei Chen, Kerstin Fauland, Walter Keller, Petra Zieglmayer, RenÃ© Zieglmayer, et al.mM DTT, 0.01 mM NaN3, 10 mg/L PMSF) to continue purification by hydroxypatite chromatography using a Bio-Gel HT Hydroxyapatite 5 ml column (BioRad, Hercules, CA, USA). Solution D (300 mM Na2HPO4, pH 7, 0.25 mM DTT, 0.01 mM NaN3,

(7) Gene-Specific H1 Eviction through a Transcriptional Activatorâ†’p300â†’NAP1â†’H1 PathwayMolecular CellApril 1, 2019Miho Shimada, Wei-Yi Chen, Tomoyoshi Nakadai, Takashi Onikubo, Mohamed Guermah, Daniela Rhodes, Robert G. Roederthe extract by HCl-precipitation, purified from the supernatant by SP-Sepharose (GE healthcare) and Bio-gel HT hydroxyapatite (Bio-Rad) chromatography. FLAG-tagged Gal4-VP16, NF-κB (p50/p65), SP1 and p300 were purified as described

(8) The Initial Response of a Eukaryotic Replisome to DNA DamageMolecular CellJune 21, 2018Martin R.G. Taylor, Joseph T.P. Yeelesand released by cleavage with GST-tagged 3C protease. The eluted protein was further purified using a Bio-Gel HT hydroxyapatite column (Bio-Rad).Cdt1-Mcm2-7 – Cdt1-Mcm2-7 with a CBP-TEV tag on the N terminus of Mcm3 was purified

(9) Heterologous Expression of a Bioactive Î²-Hexosyltransferase, an Enzyme Producer of Prebiotics, from Sporobolomyces singularisApplied and Environmental MicrobiologyFebruary 15, 2013Suzanne F. Dagher, M. Andrea Azcarate-Peril, JosÃ© M. Bruno-BÃ¡rcenafractions were pooled, concentrated, resuspended in 10 mM sodium phosphate (pH 6.8), and then applied to a Bio-Gel HT hydroxyapatite column (Bio-Rad, Richmond, CA) (1/10, 2 ml) pre-equilibrated with the same buffer, washed with 10 mM

(10) Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergyClinical & Experimental AllergyOctober 1, 2011Y. Resch et al.were pooled, dialysed against 10 mm Na2HPO4, pH 7, 0.25 mm DTT, loaded onto a column prepacked with Bio-Gel HT Hydroxyapatite (BioRad, Hercules, CA, USA) and eluted by continuously increasing the concentration of Na2HPO4 to 300

(11) Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human PlasmaJournal of Biomedicine and BiotechnologyMarch 8, 2011Teresa R. McCurdy, Varsha Bhakta, Louise J. Eltringham-Smith, Sharon Gataiance, Alison E. Fox-Robichaud, William P. Sheffieldchromatographic step. 2. Materials and Methods 2.1. Materials. Aﬃ-Gel Blue resin (150–300 μm particle size), Bio-Gel HT hydroxyapatite resin, CHT Ceramic hydroxyapatite resin, and Bio-Beads SM-2 adsorbent were from Bio-Rad Laboratories

(12) Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodellingThe EMBO JournalJuly 16, 2001A Eberharter, S Ferrari, G LÃ¤ngst, T Straub, A Imhof, P Varga-Weisz, M Wilm, P B BeckerAmersham-Pharmacia (Mono Q HR 5/5, Superose HR 10/30, Superdex S-200) and from BioRad (Bio-Rex 70, Bio-Gel HT hydroxyapatite). Extractions, dialyses and chromatographies were carried out at 4°C. Crude nuclear extract was prepared

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